Unlike in the first movie, Gwen Stacy is basically a second main character here, and Rio and Jess Drew have pivotal roles, improving the gender balance. In smaller roles, Spider-Man India is voiced by Karan Soni, who's Indian American Peni Parker is voiced by Kimiko Glenn, who's biracial White and Japanese and Sun-Spider is voiced by comic Danielle Perez, who's disabled and queer. The head Spider-Man in charge, Miguel O'Hara (Oscar Isaac), is cued as Latino (he speaks Spanish with Miles), and Miles' mom, Rio (Luna Lauren Velez), is Puerto Rican. Other Black characters include Miles' father and uncle, Jess Drew (Issa Rae), Hobie Brown (Daniel Kaluuya), and Margo Kess (Amandla Stenberg).
Main character Miles Morales, who's Puerto Rican and Black (and is voiced by Jamaican American actor Shameik Moore) is the first Afro-Latino superhero to headline a Marvel movie.